Hepatic Cytochrome P450 as Biomarkers of Cypermethrin Toxicity in Freshwater Teleost, Channa punctatus (Bloch)
نویسندگان
چکیده
In this study, Channa punctatus was treated with sub-lethal concentration of cypermethrin (6.6 μg/L) for 5, 10 and 15 days and its effect on total CYP 450 and the activity of hepatic CYP450 isoforms measured. Total CYP450 content and CYP1A mediated EROD activity was significantly induced (p<0.05) in all three treated groups compared to control whereas only 15 days treated group showed significant induction in CYP2B mediated N,Ndimethylaniline demethylase activity. CYP2E1 mediated aniline hydroxylase activity showed only a marginal increase while there was inhibition of CYP3A4 mediated erythromycin demethylase activity. Liver somatic index (LSI) also showed a marginal increase in all the treated groups. Results showed differential induction of CYP1A, CYP2B, CYP2E1 and inhibition of CYP3A4 isoform due to cypermethrin treatment in C. punctatus. The study clearly showed CYP1A isoform as the most responsive and important biomarker for monitoring the aquatic
منابع مشابه
Transmission electron microscopic study of gills of freshwater fish Channa punctatus (Bloch) exposed to the toxicity of cypermethrin
In the present study, an attempt has been made to examine the toxic effect of cypermethrin, a synthetic pyrethroid exposed to freshwater fish Channa punctatus. The fishes were exposed to sublethal concentration of 0.08 mg/L for 15, 30 and 45 days. The gills of the fish plays vital role in respiration and are an important organ with specific functions which are highly sensitive to many factors. ...
متن کاملBiomarkers of monocrotophos in a freshwater fish Channa punctatus (Bloch).
Activity of a few biomarkers have been investigated on freshwater fish Channa punctatus treated with monocrotophos for acute exposure to 18.56 ppm at 96 hr and subacute exposure viz. 0.46 ppm, 0.96 ppm and 1.86 ppm for 30 days. Biomarkers such as total protein, lipid peroxidation and acetylcholinesterase have been measured in different tissues of fish viz. gills, liver, brain and muscles. The p...
متن کاملDevicyprin induced gonadal impairment in a freshwater food fish, Channa punctatus (Bloch).
Synthetic pyrethroids are the recent major class of broad spectrum, photostable, organic insecticides used in agricultural, domestic and veterinary applications and now account for more than 30% of global insecticide use. Cypermethrin is metabolized and eliminated significantly more slowly by fish than by mammals or birds, which may explain this compound's high toxicity in fish compared to othe...
متن کاملStudies on histopathological changes in the gill, liver and kidney of Channa punctatus (Bloch) exposed to Alachlor.
Freshwater fish, Channa punctatus was exposed to sublethal concentration of a chloroacetanilide herbicide alachlor technical grade and lasso 50% EC for a period of 10 days. The histopathological changes in the gill include: necrosis, vacuolar degeneration, fusion and atrophy of primary and secondary gill lamellae. The tissue damages like degeneration of cytoplasm in hepatocytes, atrophy formati...
متن کاملToxic Impact of Nuvan (DDVP) on Tissues of Common Carp Channa punctatus (Bloch.)
Nuvan (DDVP) is a member of organophosphorus compounds. It is extremely toxic to insects and is widely used as insecticides. Nuvan has been taken for the acute toxicity to a fresh water fish Channa punctatus LC50 value calculated for the acute toxicity, which were 0.024 ml/L. Histopathological study of kidney and liver after 24hr, 48hr, 72hr and 96hr exposure to acute toxicity of Nuvan. Treatme...
متن کامل